Dithranol downregulates expression of Id1 mRNA in human keratinocytes in vitro.

Effects of the antipsoriatic drug dithranol on E2A and caspase-9 gene expression in vitro.

Although the precise causes of psoriasis remain to be elucidated, psoriasis has been known as a disorder in which factors in the immune system, enzymes and other biochemical substances that regulate skin cell division are functionally imbalanced, thereby resulting in rapid proliferation of keratinocytes and incomplete keratinization. The expression of candidate genes such as E2A and caspa...

Isolation and in vitro cultivation of human skin Keratinocytes and preparation of epidermal sheet

Comprehensive analysis of zinc derivatives pro-proliferative, anti-apoptotic and antimicrobial effect on human fibroblasts and keratinocytes in a simulated, nutrient-deficient environment in-vitro

Zinc as therapeutic agent in skin and wound care has been known for centuries, but its role is controversial and comprehensive investigations in nutrient-deficient environments are lacking. We aimed to provide a broad analysis of different zinc derivatives on proliferation, apoptosis and antimicrobial properties in a simulated nutrient-deficient environment in-vitro. Human fibroblasts (CRL2522)...

In vitro Co-Culture of Human Skin Keratinocytes and Fibroblasts on a Biocompatible and Biodegradable Scaffold

Background: Extensive full-thickness burns require replacement of both epidermis and dermis. In designing skin replacements, the goal has been to re-create this model and make a product which has both essential components. Methods: In the present study, we developed procedures for establishing confluent, stratified layers of cultured human keratinocytes on the surface of modified collagen-chito...

P-30: The Investigation of Transcript Expression Level of Mitochondrial Transcription Factor A (TFAM) during In Vitro Maturation (IVM) in Single Human Oocytes

Background In vitro maturation (IVM) of human oocytes has acquired increasing attention in infertility treatment with great promise. This technique is an alternative conventional in vitro fertilization-embryo transfer (IVF-ET), and can be reduced the side effects of gonadotropin stimulation such as ovarian hyperstimulation (OHSS). Oocyte maturation is a complex process including cytoplasmic and...